The MOOC of Cell Biology Experiments aims to improve basic knowledge and experimental skills and introduce cutting-edge applications of cell biology to students. This course is mainly composed of eight themes, including five themes of basic experimental methods and three themes of practical cutting-edge research technologies. The first five themes of basic experimental methods are: the use of microscopes, cell specimen preparation, cell acquisition and cell separation, cell culture , cell viability assay. The practical cutting-edge research technologies include: apoptosis detection, autophagy detection, cell migration and invasion assays. Each experimental chapter is divided into three parts: method overview, experiment demonstration and analysis of experimental results. Before experimental teaching, two special opening lectures are presented to enhance your learning interest and broaden your insights.
The education ideas of this MOOC are based on comprehensive consideration of all aspects of learners’ requirements. The contents are from basic to comprehensive, from classic to frontier, and from method to application in order to improve the learners’ scientific research capacity.
The course teachers have plenty of experiences in teaching and are engaged in cell biology teaching and research for many years. They focus on teaching research and the construction of digital teaching materials. In the experimental teaching of cell biology, they can provide good instructions with experience accumulated for decades. The course is filmed in the National Demonstration Center for Life Sciences Experiment Education, Nanjing University.
Hope you have a wonderful learning experience and gain a lot of knowledge from this course.
Lecture 1: Application of Cell Biology Experimental Technology in disease treatment
Lecture 2: Application of Cell Biology Experimental Technology in drug development
Part One: Microscopes and Its Use
1.1 Overview of microscope
1.2 Structure and use method of ordinary optical microscope ( Operation demo)
1.3 Structure and use method of inverted fluorescence microscope ( Operation demo)
Part Two: Cell Specimen Preparation
2.1 Overview of cell specimen preparation technique
2.2 Blood smear: preparation and staining ( Operation demo)
2.3 Preparation of onion root-tip cell specimen by tapping ( Operation demo)
2.4 Preparation of specimen of plant tissue with hand-held slicing ( Operation demo)
2.5 Preparation of frozen tissue sections ( Operation demo)
2.6 Experiment result analysis for blood smear
2.7 Experiment result analysis for onion root tip
Part Three: Cell Acquisition and Cell Separation
3.1 Overview of cell acquisition and cell isolation methods
3.2 Isolation of mouse spleen mononuclear cells ( Operation demo)
3.3 Preparation of plant protoplasts by enzymolysis ( Operation demo)
3.4 Separation of peripheral blood mononuclear cells ( Operation demo)
3.5 Acquisition of mouse peritoneal macrophages ( Operation demo)
3.6 Experiment result analysis for mononuclear cell isolation
3.7 Experiment result analysis for plant protoplasts separation
Part Four: Cell Culture
4.1 Introduction
4.2 Primary cell culture ( Operation demo)
4.3 Passaging of cultured cells ( Operation demo)
4.4 Cell cryopreservation ( Operation demo)
4.5 Experiment result analysis for cell culture
Part Five: Cell Viability Assay
5.1 Overview of cell viability assay
5.2 Cell counting
5.2.1 Experimental principle and operation ( Operation demo)
5.2.2 Experiment result analysis and notes
5.3 MTT assay
5.3.1 Experimental principle and operation( Operation demo)
5.3.2 Experiment result analysis and notes
Part Six: Apoptosis Detection
6.1 Overview of apoptosis dectection methods
6.2 Cell morphology observation
6.2.1 Experimental principle and operation ( Operation demo)
6.2.2 Experiment result analysis and notes
6.3 FITC-AnnexinV / PI Apoptosis detection
6.3.1 Apoptosis detection by fluorescence microscope ( Operation demo)
6.3.2 Apototosis detection by flow cytometry ( Operation demo)
6.3.3 Experiment result analysis and notes
Part Seven: Autophagy Detection
7.1 Overview of autophagy detection methods
7.2 LC3-GFP Reporter
7.2.1 LC3-GFP transfection ( Operation demo)
7.2.2 Autophagy detection by fluorescence microscopy ( Operation demo)
7.2.3 Experiment result analysis and notes
Part Eight: Cell Migration and Invasion Assays
8.1 Overview of cell migration and invasion assays
8.2 Wound Healing assay
8.2.1 Experimental principle and operation ( Operation demo)
8.2.2 Experiment result analysis and notes
8.3 Transwell assay
8.3.1 Experimental principle and operation ( Operation demo)
8.3.2 Experiment result analysis and notes